human cd200 non-lytic recombinant protein fc tag lyophilized (Innovative Research Inc)

N/A

Human CD200 non-lytic Recombinant Protein Fc Tag Lyophilized from Innovative Research has been recombinantly produced in CHO cells. This is a Lyophilized protein buffered in Lyophilized from 0.2um-filtered solution in PBS. Reconstitute at 100 and#956;g/ml

Buy from Supplier

recombinant human cd200 fc (R&D Systems)

R&D Systems recombinant human cd200 fc
Recombinant Human Cd200 Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+cd200+fc+chimera+protein/Recombinant+Human+CD200+Fc+Chimera+Protein%2C+CF/pmc03446504-35-47-61
Average 91 stars, based on 1 article reviews

recombinant human cd200 fc - by Bioz Stars, 2026-10

91/100 stars

Buy from Supplier

recombinant human cd200 fc chimera protein (R&D Systems)

R&D Systems recombinant human cd200 fc chimera protein

Figure 1. Recombinant <t>CD200</t> inhibits osteoclast formation. (A) Adherent peripheral blood mononuclear cells (PBMCs) were cultured with M-CSF for 48 hr, RANKL was added on day 3 of culture and medium was changed every 3 days. <t>rCD200</t> was added at the beginning of the culture and every 3 days. After 21 days of culture, osteoclast formation was determined by the use of tartrate-resistant acid phosphatase (TRAP) assay. Data are mean±SD area of osteoclasts from 3 independent experiments. (B) rCD200 inhibits bone resorption activity. PBMCs were plated on bone slices in wells of 96-well plates and stimulated as described. After 21 days of culture, resorbtion pitt number and area were count under a light microscope (arrows). **, P<0.01 compared with none; *, P<0.05 compared with M+R alone.

Recombinant Human Cd200 Fc Chimera Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+cd200+fc+chimera+protein/Recombinant+Human+CD200+Fc+Chimera+Protein%2C+CF/pm23940819-63-0-9
Average 93 stars, based on 1 article reviews

recombinant human cd200 fc chimera protein - by Bioz Stars, 2026-10

93/100 stars

Buy from Supplier

recombinant human cd200 fc chimera protein cf (R&D Systems)

N/A

The Recombinant Human CD200 Fc Chimera Protein from R D Systems is derived from NS0 The Recombinant Human CD200 Fc Chimera Protein has been validated for the following applications Binding Activity

Buy from Supplier

recombinant human cd200 r1 fc chimera protein cf (R&D Systems)

N/A

The Recombinant Human CD200 R1 Fc Chimera Protein from R D Systems is derived from NS0 The Recombinant Human CD200 R1 Fc Chimera Protein has been validated for the following applications Binding Activity

Buy from Supplier

human cd200 recombinant protein c-fc tag lyophilized (Innovative Research Inc)

N/A

Human CD200 Recombinant Protein C-Fc Tag Lyophilized from Innovative Research has been recombinantly produced in Human Cells. This is a Lyophilized protein buffered in Lyophilized from a 0.2 um filtered solution of 20mM PB,150mM NaCl,pH7.4.

Buy from Supplier

human cd200 receptor 1 recombinant protein c-fc tag lyophilized (Innovative Research Inc)

N/A

Human CD200 Receptor 1 Recombinant Protein C-Fc Tag Lyophilized from Innovative Research has been recombinantly produced in Human Cells. This is a Lyophilized protein buffered in Lyophilized from a 0.2 um filtered solution of 20mM

Buy from Supplier

human cd200 active recombinant protein c-his & fc tag lyophilized (Innovative Research Inc)

N/A

Human CD200 Recombinant Protein C-His & Fc Tag Lyophilized from Innovative Research has been recombinantly produced in HEK293 Cells. The protein formulation is lyophilized from sterile PBS; pH 7.4 with a purity of > 95

Buy from Supplier

recombinant human cd200 fc chimera avi-tag protein, cf (R&D Systems)

N/A

Recombinant Human CD200 Fc Chimera Avi-tag Protein, CF

Buy from Supplier

Image Search Results

Figure 1. Recombinant CD200 inhibits osteoclast formation. (A) Adherent peripheral blood mononuclear cells (PBMCs) were cultured with M-CSF for 48 hr, RANKL was added on day 3 of culture and medium was changed every 3 days. rCD200 was added at the beginning of the culture and every 3 days. After 21 days of culture, osteoclast formation was determined by the use of tartrate-resistant acid phosphatase (TRAP) assay. Data are mean±SD area of osteoclasts from 3 independent experiments. (B) rCD200 inhibits bone resorption activity. PBMCs were plated on bone slices in wells of 96-well plates and stimulated as described. After 21 days of culture, resorbtion pitt number and area were count under a light microscope (arrows). **, P<0.01 compared with none; *, P<0.05 compared with M+R alone.

Journal: PloS one

Article Title: CD200R/CD200 inhibits osteoclastogenesis: new mechanism of osteoclast control by mesenchymal stem cells in human.

doi: 10.1371/journal.pone.0072831

Figure Lengend Snippet: Figure 1. Recombinant CD200 inhibits osteoclast formation. (A) Adherent peripheral blood mononuclear cells (PBMCs) were cultured with M-CSF for 48 hr, RANKL was added on day 3 of culture and medium was changed every 3 days. rCD200 was added at the beginning of the culture and every 3 days. After 21 days of culture, osteoclast formation was determined by the use of tartrate-resistant acid phosphatase (TRAP) assay. Data are mean±SD area of osteoclasts from 3 independent experiments. (B) rCD200 inhibits bone resorption activity. PBMCs were plated on bone slices in wells of 96-well plates and stimulated as described. After 21 days of culture, resorbtion pitt number and area were count under a light microscope (arrows). **, P<0.01 compared with none; *, P<0.05 compared with M+R alone.

Article Snippet: Recombinant human CD200 Fc chimera protein (rCD200) was from R&D Systems (Minneapolis, MN, USA).

Techniques: Recombinant, Cell Culture, TRAP Assay, Activity Assay, Light Microscopy

Figure 4. Within MSCs, CD200+ population inhibits osteoclast formation. PBMC adherent cells were co-cultured with MSCs in presence of M-CSF for 48 hr. RANKL was added after 48 hr and half of the medium was changed every 3 days. (A) After 21 days of culture, actin rings were stained with phalloidin-Alexa 540 and nuclei with DAPI. (B) Osteoclast precursors were cultivated with MSCs, CD200+ MSCs or CD200– MSCs for 21 days. Tartrate-resistant acid phosphatase (TRAP) assay was performed and area of TRAP-positive cells was measured. Data are mean±SD. *, P<0.05. (C) Effect of MSCs on bone resorption activity of osteoclasts. White arrows represent bone resorption pits. The results of 1 experiment out of 3 are presented.

Journal: PloS one

Article Title: CD200R/CD200 inhibits osteoclastogenesis: new mechanism of osteoclast control by mesenchymal stem cells in human.

doi: 10.1371/journal.pone.0072831

Figure Lengend Snippet: Figure 4. Within MSCs, CD200+ population inhibits osteoclast formation. PBMC adherent cells were co-cultured with MSCs in presence of M-CSF for 48 hr. RANKL was added after 48 hr and half of the medium was changed every 3 days. (A) After 21 days of culture, actin rings were stained with phalloidin-Alexa 540 and nuclei with DAPI. (B) Osteoclast precursors were cultivated with MSCs, CD200+ MSCs or CD200– MSCs for 21 days. Tartrate-resistant acid phosphatase (TRAP) assay was performed and area of TRAP-positive cells was measured. Data are mean±SD. *, P<0.05. (C) Effect of MSCs on bone resorption activity of osteoclasts. White arrows represent bone resorption pits. The results of 1 experiment out of 3 are presented.

Article Snippet: Recombinant human CD200 Fc chimera protein (rCD200) was from R&D Systems (Minneapolis, MN, USA).

Techniques: Cell Culture, Staining, TRAP Assay, Activity Assay

recombinant human cd200 fc chimera protein Search Results

Image Search Results